central materials laboratory testing manual 2000

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Trichinella ChLIA detects antigens of 1 larva in 100 g infected pork (0.01 lpg).

Objective evaluation via Trichinella ChLIA allows reliable meat inspection.

Meat inspection is faster with the new method than with the gold standard.

Abstract The parasitic roundworm Trichinella spiralis is most commonly transmitted to humans through consumption of raw or undercooked meat of infected pigs or game.

To prevent human infection, slaughterhouses perform meat safety surveillance using the gold standard Magnetic Stirrer Method.

We introduce a fast and objective method using automated detection of specific Trichinella spiralis antigens by a newly developed immunoassay based on chemiluminescence (ChLIA).

Panel A comprised muscle tissue samples from non-infected pigs (n 37).

Panel B comprised muscle tissue samples from non-infected pigs spiked with different amounts of Trichinella larvae without collagen capsules (n 56).

Panel C contained muscle tissue samples from experimentally infected pigs including Trichinella larvae encapsulated in collagen (n 32).

Each sample was shredded with PBS buffer in a knife mill, destroying Trichinella larvae.

The overall accuracy of the Trichinella ChLIA was 97.6.

The specificity of the Trichinella ChLIA was 100 (panel A).

The sensitivity in samples from experimentally infected pigs was 100 representing a detection limit of 0.01 larvae per gram.

Cross-reactivity with parasites other than Trichinella spp.

In contrast to the gold standard, this new approach to meat safety surveillance does not require longsome digestion or microscopy by trained personnel.

Graphical abstract Download: Download full-size image Previous article in issue Next article in issue Keywords Antigen detection Chemiluminescence Digestion Immunoassay Meat inspection Microscopy Muscle larvae Pig Surveillance Trichinella spiralis Recommended articles Citing articles (0) 2020 The Author(s).

Published by Elsevier B.V.

Central Materials Laboratory Testing Manual 2000